Publications Featuring LabeoTech
A Longitudinal Pilot Study on Cognition and Cerebral Hemodynamics in a Mouse Model of Preeclampsia Superimposed on Hypertension: Looking at Mothers and Their Offspring
Trigiani, L. J., Lecrux, C., Royea, J., Lavoie, J. L., Lesage, F., Pilote, L., & Hamel, E. (2021). Preeclampsia is a common hypertensive disorder in pregnant women and whose causes and consequences have focused primarily on cardiovascular outcomes on the mother and offspring, often without taking into consideration the possible effects on the brain. One possible cause of preeclampsia has been att…
Developmental and interventional plasticity of motor maps after perinatal stroke
Zhang, S. Y., Jeffers, M. S., Lagace, D. C., Kirton, A., & Silasi, G. (2021). Within the perinatal stroke field, there is a need to establish preclinical models where putative biomarkers for motor function can be examined. In a mouse model of perinatal stroke, we evaluated motor map size and movement latency following optogenetic cortical stimulation against three factors of post-stroke biomarker utility: 1) Correlation to chronic impairment on a behavioral test battery; 2) Amenability to change using a …
Mesoscopic Mapping of Stimulus-Selective Response Plasticity in the Visual Pathways Modulated by the Cholinergic System
Laliberté, G., Othman, R., & Vaucher, E. (2020). The cholinergic potentiation of visual conditioning enhances visual acuity and discrimination of the trained stimulus. To determine if this also induces long-term plastic changes on cortical maps and connectivity in the visual cortex and higher associative areas, mesoscopic calcium imaging was perfo…
A Pilot Study Investigating Changes in Capillary Hemodynamics and Its Modulation by Exercise in the APP-PS1 Alzheimer Mouse Model
Lu, X., Moeini, M., Li, B., Lu, Y., Damseh, R., Pouliot, P., … & Lesage, F. (2019). Dysfunction in neurovascular coupling that results in a mismatch between cerebral blood flow and neuronal activity has been suggested to play a key role in the pathogenesis of Alzheimer’s disease (AD). Meanwhile, physical exercise is a powerful approach for maintaining cognitive health and could pla…
Restored immune cell functions upon clearance of senescence in the irradiated splenic environment – PubMed
Palacio, L., Goyer, M. L., Maggiorani, D., Espinosa, A., Villeneuve, N., Bourbonnais, S., … & Beauséjour, C. (2019). Some studies show eliminating senescent cells rejuvenate aged mice and attenuate deleterious effects of chemotherapy. Nevertheless, it remains unclear whether senescence affects immune cell function. We provide evidence that exposure of mice to ionizing radiation (IR) promotes the senescent-associat…
The tumor immune response is not compromised by mesenchymal stromal cells in humanized mice
Moquin-Beaudry,G., Colas, C., Li, Y., Bazin, R., Jean V. Guimond, Haddad,E., and Beauséjour,C. (2019). Therapeutic uses of mesenchymal stromal cells (MSCs) have emerged over the past decade. Yet, their effect on tumor growth remains highly debated, particularly in an immune competent environment. Here, we wanted to investigate the impact of human umbilical cord MSCs (hUC-MSCs) on tumor growth in huma…
INK4a/ARF Expression Impairs Neurogenesis in the Brain of Irradiated Mice
Le,O., Palacio,L., Bernier,G., Batinic-Haberle,I., Hickson,G., and Beauséjour,C. (2018). Brain neurogenesis is severely impaired following exposure to ionizing radiation (IR). We and others have shown that the expression of the tumor suppressor gene p16INK4a is increased in tissues exposed to IR and thus hypothesized that its expression …
Development of a new bioactivatable fluorescent probe for quantification of apolipoprotein A-I proteolytic degradation in vitro and in vivo
F. Maafi, B. Li, C. Gebhard, M. Brodeur, L. Villeneuve, F. Lesage, D. Rhainds, E. Rhéaume, and J.-C. Tardif. (2017). The potential benefits of high-density lipoproteins (HDL) against atherosclerosis are attributed to its major protein component, apolipoprotein A-I (a…
Impact of hMSCs on solid tumor growth in novel tumor/immune system humanized mice
Moquin-Beaudry,G., Desaulniers-Langevin,C., Benabdallah,B., Palacio,L., Beauséjour,C. (2017). Clinical trial approbation for novel solid tumor therapies have been steadily decreasing since the 1990s to reach barely 7% in recent years constituting considerable health and financial concerns that need to be addressed. We believe one of the reasons behind such worrying number is the lack of clinically relevant and accessible pre-clinical models notably for the study of the patient’s own immune system on tumor emergence, progression and elimination.
In vitro differentiated plasmacytoid dendritic cells as a tool to induce anti-leukemia activity of natural killer cells – Cancer Immunology, Immunotherapy
Díaz-Rodríguez, Y. Cordeiro,P., Belounis,A., Herblot,S., and Duval,M. (2017). Acute lymphoblastic leukemia (ALL) is believed to be resistant to NK cell-mediated killing. To overcome this resistance, we developed an innovative approach based on NK cell stimulation with Toll-like receptor (TLR)-activated plasmacytoid dendritic cells (pDC). The translation of this approach into …
L. Palacio, S. Bourbonnais, N. Villeneuve, G. Moquin-Beaudry, O. Le, H. Decaluwe, C. Beauséjour, IR-induced senescence of the splenic microenvironment impacts murine immune cells functions, International Cell Senescence Association (ICSA) Conference 2017, Paris, France.
Colas C, Li Y*, Dicaire R*, Guimond J, Patey N, Vobecky S, Haddad E: Generation of functional human T-cell development in NOD/SCID/IL2rγnull humanized mice without using fetal tissue. 5th International Workshop on Humanized Mice, January 28-30, 2016 Zurich, Switzerland
Colas C, Li Y*, Dicaire R*, Guimond J, Patey N, Haddad E: Optimization of the humanized NOD/SCID/IL2rγnull Bone marrow-Liver-Thymus (BLT) mouse model. 5th International Workshop on Humanized Mice, January 28-30, 2016 Zurich, Switzerland
Colamartino A, Nicoletti S, Herblot S, Duval M, Haddad E : New approach in immunotherapy of acute lymphoblastic leukemia. 29th Annual Spring Meeting of the Canadian Society for Immunology, April 1–4, 2016 Ottawa, ON, Canada
Moquin-Beaudry,G., Desaulniers-Langevin,C., Benabdallah,B., Palacio,L., Beauséjour,C., IMPACT OF hMSCs ON SOLID TUMOR GROWTH IN NOVEL TUMOR/IMMUNE SYSTEM HUMANIZED MICE, 2016 ISSCR meeting, June 22-25 2016, San Francisco, CA, United States.
Palacio,L., Lee,O., Moquin-Beaudry,G., Bourbonnais,S., Beauséjour,C. IR-induced p16INK4a expression in the splenic niche modulates the immune system. XXXIe Congrès des étudiants gradués et post-gradués du centre de recherche du CHU Ste-Justine, 2016, Montreal, Canada.
Palacio,L., Lee,O., Moquin-Beaudry,G., Bourbonnais,S., Beauséjour,C. IR-induced p16INK4a expression in the splenic niche modulates the immune system. XXI Journée de la recherche Gabriel L. Plaa, 2016, Montreal, Canada.
Moquin-Beaudry,G., Palacio,L., Bardeau,P., Benabdallah,B., Lee,O., Beauséjour,C. Development and applications for a novel human autologous tumor/immune system xenograft model in mice, 2015 Canadian Cancer Research Conference, November 8-10 2015, Montreal, QC, Canada.
Moquin-Beaudry,G., Palacio,L., Benabdallah,B., Beauséjour,C. Impact of hUC-MSCs on tumor growth in a humanized autologous tumor/immune system xenograft model in mice, 2015 Montreal Immunology Meeting, November 23 2015, Montreal, QC, Canada.
Palacio,L., Lee,O., Beauséjour,C. IR-induced p16INK4a expression in the spleen impairs immune cell functions,Montreal Immunology Meeting (MIM), 2015, Montreal, Canada.
Palacio,L., Lee,O., Beauséjour,C. L’expression du gène suppresseur de tumeur p16INK4a dans le microenvironnement tumoral joue en rôle important dans la réponse thérapeutique chez les souris. XX Journée de la recherche Gabriel L. Plaa, 2015, Montreal, Canada.
Palacio,L., Lee,O., Beauséjour,C. L’expression du gène suppresseur de tumeur p16INK4a dans le microenvironnement tumoral joue en rôle important dans la réponse thérapeutique chez les souris. XXX congrès des étudiants gradués et post-gradués du centre de recherche du CHU Ste-Justine, 2015, Montreal, Canada.
Hybrid FMT-MRI applied to in vivo atherosclerosis imaging
B. Li, F. Maafi, R. Berti, P. Pouliot, and F. Lesage. (2014). Combining Fluorescent Molecular Tomography (FMT) with anatomical imaging, e.g. MRI facilitates interpreting functional information. Furthermore, using a heterogeneous model for light propagation has been shown in simulations to be superior to homogeneous modeling to quantify fluorescence. Here, we p…
MRI-guided fiber-based fluorescence molecular tomography for preclinical atherosclerosis imaging
B. Li, P. Pouliot, F. Lesage. (2014). Multi-modal imaging combining fluorescent molecular tomography (FMT) with MRI could provide information in these two modalities as well as optimize the recovery of functional information with MR-guidance. Here, we present a MRI-guided FMT system. An optical probe was designed consisting of a fiber plate on the top and bottom sides of the animal bed, respectively.
Dopamine D1 receptor signalling in dyskinetic Parkinsonian rats revealed by fiber photometry using FRET-based biosensors
Jones-Tabah, J., Mohammad, H., Hadj-Youssef, S., Kim, L. E., Martin, R. D., Benaliouad, F., … & Hébert, T. E. (2020). As with many G protein-coupled receptors (GPCRs), the signalling pathways regulated by the dopamine D1 receptor (D1R) are dynamic, cell type-specific, and can change in the face of disease or drug exposures. In striatal neurons, the D1R activates cAMP/protein …
Physiological Monitoring System
Sparse channel sampling for ultrasound localization microscopy (SPARSE-ULM) – PubMed
Hardy, E., Porée, J., Belgharbi, H., Bourquin, C., Lesage, F., & Provost, J. (2021). Ultrasound localization microscopy (ULM) has recently enabled the mapping of the cerebral vasculaturein vivowith a resolution ten times smaller than the wavelength used, down to ten microns. However, with frame rates up to 20000 frames per second, this method requires large amount of data to …
Spatio-temporal patterns of population responses in the visual cortex under Isoflurane: from wakefulness to loss of consciousness
Margalit, S. N., Golomb, N. G., Tsur, O., Raz, A., & Slovin, H. (2020). Anesthetic drugs are widely used in medicine and research to mediate loss of consciousness (LOC). Despite the vast use of anesthesia, how LOC affects cortical sensory processing and the underlying neural circuitry, is not well understood. We measured neuronal population activity in the visual cortic…
Progression of regional lung strain and heterogeneity in lung injury: assessing the evolution under spontaneous breathing and mechanical ventilation – Annals of Intensive Care
Hurtado, D. E., Erranz, B., Lillo, F., Sarabia-Vallejos, M., Iturrieta, P., Morales, F., … & Cruces, P. (2020). Background Protective mechanical ventilation (MV) aims at limiting global lung deformation and has been associated with better clinical outcomes in acute respiratory distress syndrome (ARDS) patients. In ARDS lungs without MV support, the mechanisms and evolution of lung tissue deformation remain un…
Longitudinal optical coherence tomography imaging of tissue repair and microvasculature regeneration and function after targeted cerebral ischemia
Lu, Y., Lu, X., Zhang, C., Marchand, P. J., & Lesage, F. (2020). Stroke survivors are often left with serious, long-term disabilities, such as reduced mobility, declined cognition, and impaired sensation, due to permanent neurological damage to the brain. Evidence from animal models and human subjects both show that the brain has a certain degree of spontaneous plasticity after stroke,1,2 and a maximal functional recovery can be achieved in a time-limited plastic window by harnessing neuroplasticity.
Intracellular calcium mishandling leads to cardiac dysfunction and ventricular arrhythmias in a mouse model of propionic acidemia
Tamayo, M., Fulgencio-Covian, A., Navarro-Garcia, J. A., Val-Blasco, A., Ruiz-Hurtado, G., Gil-Fernández, M., … & Richard, E. (2020). Propionic acidemia (PA) is a rare metabolic disease associated with mutations in genes encoding the α and β subunits of the enzyme propionyl-CoA carbo…
Beneficial effects of paricalcitol on cardiac dysfunction and remodelling in a model of established heart failure
Tamayo, M., Martín-Nunes, L., Val-Blasco, A., GM-Piedras, M. J., Navarro-García, J. A., Lage, E., … & Delgado, C. (2020). Chronic heart failure (HF) is a major public health concern in ageing societies and a leading cause of hospitalisation and mortality (Ponikowski et al., 2014; Savarese & Lund, 2017). The disorder is usually accompanied by progression from adaptive to maladaptive hypertrophy and also cardiac dilation and diminished left ventricular ejection fraction (LVEF).
Mapping regional strain in anesthetised healthy subjects during spontaneous ventilation
Cruces, P., Erranz, B., Lillo, F., Sarabia-Vallejos, M. A., Iturrieta, P., Morales, F., … & Hurtado, D. E. (2019). Introduction Breathing produces a phenomenon of cyclic deformation throughout life. Biomechanically, deformation of the lung is measured as strain. Regional strain recently started to be recognised as a tool in the study of lung pathophysiology, but regional lung strain has not been studied in healt…
Evaluation of upconverting nanoparticles towards heart theranostics
Kermorgant, M., Ben Salem, J., Santelli, J., Calise, D., Oster, A. C., Lairez, O., … & Beaudry, F. (2019). Restricted and controlled drug delivery to the heart remains a challenge giving frequent off-target effects as well as limited retention of drugs in the heart. There is a need to develop and optimize tools to allow for improved design of drug candidates for treatment of heart diseases. Over the last…
Effects of Landiolol in Lipopolysaccharide-Induced Acute… : Shock
Kiyonaga, N., Moriyama, T., & Kanmura, Y. (2019). Septic acute kidney injury (AKI) is one of the most common complications and is associated with high mortality. Sepsis or septic shock is the greatest risk factor causing AKI in patients in intensive care units, and it is reported that the cause of about 30% to 70% of AKI cases is sepsis (1–5). To date, no drug has been shown to confer renal protection in the clinical setting.
Increased glymphatic influx is correlated with high EEG delta power and low heart rate in mice under anesthesia
Hablitz, L. M., Vinitsky, H. S., Sun, Q., Stæger, F. F., Sigurdsson, B., Mortensen, K. N., … & Nedergaard, M. (2019). The glymphatic system is responsible for brain-wide delivery of nutrients and clearance of waste via influx of cerebrospinal fluid (CSF) alongside perivascular spaces and through the brain. Glymphatic system activity increases during sleep or ketamine/xylazine (K/X) anesthesia, yet the mechanism(s) …
Fibroblast growth factor-23 promotes rhythm alterations and contractile dysfunction in adult ventricular cardiomyocytes
Navarro-García, J. A., Delgado, C., Fernández-Velasco, M., Val-Blasco, A., Rodríguez-Sánchez, E., Aceves-Ripoll, J., … & Praga, M. (2019). AbstractBackground. Cardiac dysfunction and arrhythmia are common and onerous cardiovascular events in end-stage renal disease (ESRD) patients, especially those
Basal Sodium-Dependent Vitamin C Transporter 2 polarization in choroid plexus explant cells in normal or scorbutic conditions – Scientific Reports
Ulloa, V., Saldivia, N., Ferrada, L., Salazar, K., Martínez, F., Silva-Alvarez, C., … & Cifuentes, M. (2019). Vitamin C is incorporated into the cerebrospinal fluid (CSF) through choroid plexus cells. While the transfer of vitamin C from the blood to the brain has been studied functionally, the vitamin C transporter, SVCT2, has not been detected in the basolateral membrane of choroid plexus cells. Furthermore, it is unknown how its expression is induced in the developing brain and modulated in scurvy conditions.
Exenatide Reduces Graft Injury in a Rat Transplantation Model Using Kidneys Donated after Cardiac Death
Yang, H., Shi, Y., Long, C., Li, X., Luo, L., Chen, M., … & Wang, Z. (2019, July). Besides being used in the therapy of type 2 diabetes, exenatide reduces cerebral ischemia-reperfusion (I/R) injury. We evaluated the potential effects…
Voluntary running enhances glymphatic influx in awake behaving, young mice
von Holstein-Rathlou, S., Petersen, N. C., & Nedergaard, M. (2018). Vascular pathology and protein accumulation contribute to cognitive decline, whereas exercise can slow vascular degeneration and improve cognitive fun…
Correlation of hemodynamic and fluorescence signals under resting state conditions in mice’s barrel field cortex
Bélanger, S., Oliveira Ferreira de Souza, B., Casanova, C., & Lesage, F. (2016). Both neurons and astrocytes are known to affect local vascular response in the brain following neuronal activity. In order to differentiate the contri…
White matter injury in a newborn rat model analyzed with Resting-State Optical Imaging of Intrinsic Signals (rsOIS)
Guevara, E., Sirpal, P., Abran, M., Pouliot, P., Lesage, F., Jin, C., … Lodygensky, G. A. (2015). Using rsOIS (Resting-State Optical Imaging of Intrinsic Signals), we demonstrated a non-invasive approach to assess cerebral changes in response to inflammatory white matter injury. Surprisingly, lipopolysaccharide (LPS) exposure induced an increase in inter-hemispheric functional connectivity (fc);…