LabeoTech

OiS200 Sales Information

Main System

ois200 on a white background

OiS200

  • Performs Intrinsic Optical Imaging, Fluorescence Imaging and/or Speckle Imaging.
  • This imaging system offers an easy setup and outstanding performance acquired via a user-friendly software.
  • sCMOS camera (1.5 e- read noise). 
  • 1024 X 1024 @ 50 fps, 512 X 512 @ 100 fps, 256 X 256 @ 200 fps, 128 X 128 @ 375 fps, 32 X 32 @ 1000 fps.
  • Exposure time down to 0.1ms. 
  • Binning and ROI selection.
  • 50 mm lens. 
  • Field of view: 8 mm X 8 mm or more (valid only without the optogenetic stimulator add-on). 
  • Manually adjustable numerical aperture f/1.2 to 16.
  • High quality steel plate allowing the use of a magnetic positioner.
  • Dimensions: 18″ (width) X 22″ (depth) X 23″ (height).
  • Multiple illumination arms fixation positions.

Imaging Options (select at least one)

intrinsic add on

Intrinsic signal optical imaging (Item #35000)

  • 3X High intensity LEDs (525 nm, 590 nm & 625 nm) for reflectance.
  • Includes Köhler optics to obtain the same spot size and position for each LED.
  • Controls integrated to the system’s software.
  • Manual intensity adjustments.
  • Easy LED positioning with articulated positioning arms.
  • Iris for spot size adjustment.
florescence imaging add on

Fluorescence imaging

  • 475 nm fluorescence (GCaMP6 & OGB-1) – Item #35010
    475 nm LED with a 472 ± 15 nm filter and 496 nm long pass filters installed in front of the camera and on the intrinsic illumination arm.
  • 590 nm fluorescence (E2-Crimson & R-GECO) and Voltage-Sensitive Dyes (VSD) – Item #35011
    590 nm LED with a 571 ± 36 nm filter and a 660 ± 26 nm filter installed in front of the camera. Cannot perform intrinsic imaging simultaneously.
  • 525 nm fluorescence (R-GECO) – Item #35013
    525 nm LED with a 540 ± 40 nm filter and a 629 ± 28 nm filter installed in front of the camera. Cannot perform intrinsic imaging simultaneously.
  • 567 nm fluorescence (R-GECO) – Item #35014
    567 nm LED with a 540 ± 40 nm filter and a 629 ± 28 nm filter installed in front of the camera. Cannot perform intrinsic imaging simultaneously.
  • 625 nm fluorescence and Voltage-Sensitive Dyes (VSD) – Item #35015
    625 nm LED with a 629 ± 28 nm filter and a 685 nm long pass filter installed in front of the camera. Cannot perform intrinsic imaging simultaneously.
  • Other LED wavelength available on request among: 455, 475, 525, 567, 590 & 625 nm
  • The system is equipped with 2 fluorescence illumination drivers. If additional illuminations are purchased, only the LED and optics will be supplied. The user will be able to use a maximum of 2 illuminations at the time and can change illuminations in a few seconds. A discount is also offered after 2 illuminations.
  • Includes focussing optics to deliver a high-power density.
  • Controls integrated to the system’s software.
  • Manual intensity adjustments.
  • Easy LED positioning with articulated positioning arms.
  • Iris for spot size adjustment.
speckle imaging add-on

Speckle Imaging (Item #35020)

  • 785 nm laser diode on 1 articulated positioning arm.
  • Also available with a 660 nm laser diode.

Communication Options

Stimulation (select 0 or 1 option per system)

  • 1 analog output – Item #31001
    ±10V, BNC connector
  • 2 analog outputs – Item #31002
    ±10V, BNC connectors
  • 4 digital outputs – Item #31004
    0-5V, BNC connectors, 4 bits code output
  • 8 digital outputs – Item #31008
    0-5V, BNC connectors, 8 bits code output

 

  • Analog stimulation:
    • Adjustable frequency, duration, pulse width, amplitude (biphasic or not), number of repetitions and inter stimulation duration with jitter (random component).
    • Real time unprocessed response display during the acquisition.
  • Digital stimulation:
    • Digital coded output and adjustable number of repetitions.
    • Real time unprocessed response display during the acquisition.

Analog Inputs (select 0 or 1 option per system)

  • 4 analog inputs – Item #32004
  • 8 analog inputs – Item #32008
  • ±10V, BNC connector, 10 kSamples/second.

Add-Ons (maximum of 1 add-on per system)

lighttrack ois200 banner

Optogenetic Stimulation Add-On

  • 450 nm laser stimulation/inhibition (Item #35050-450)
    With a 495 nm long-pass dichroic filter
  • 637nm laser stimulation/inhibition (Item #35050-637)
    With a 625 nm short-pass dichroic filter
  • 660 nm laser stimulation/inhibition (Item #35050-660)
    With a 653 nm short-pass dichroic filter
  • 589nm laser stimulation/inhibition (Item #35050-589)
    With a 593 ± 19 nm cut-band dichroic filter
  • 450 & 660 nm laser stimulation/inhibition (Item #35050-450-660)
    Easily switch from one wavelength to the other by swapping the magnetic filter mount
  • 450 & 589 nm laser stimulation/inhibition (Item #35050-450-589)
    Easily switch from one wavelength to the other by swapping the magnetic filter mount
 
  • Spot size down to 50 µm, up to 250 mW/mm2 power output.
  • 2D Illumination Scanning with galvo mirrors.
  • Co-axial optical mount to pre-existing optical imager.
  • Tandem lenses: 25 mm & 50 mm.
  • Field of view: Fixed at 10 mm X 10 mm (1024 X 1024).
  • 2X teleconverters (sold separately) can be added to obtain a 10 mm X 10 mm field of view on 512 X 512 pixels, for high speed imaging.
  • Dichroic filter to ensure compatibility with existing applications.
  • Easily switch from one wavelength to the other by swapping the magnetic filter mount.
  • Multi-points stimulation: 2 points or matrix selection.
  • Pre-conditioning with adjustable laser power and inter-stimulation duration: fixed, ramps or list.
  • Pulsed stimulation up to 500 Hz.
  • Other wavelength available on requests, multiple wavelength possible.
ois200 with spatially modulated add on

Spatially Modulated Optogenetic Stimulation Add-On

  • 450 nm 2D laser stimulation/inhibition (Item #35150-450)
    10 mW/mm2, with a 495 nm long-pass dichroic filter.
  • 520 nm 2D laser stimulation/inhibition (Item #35150-520)
    2.5 mW/mm2, with a 552 nm long-pass dichroic filter.
  • 637nm 2D laser stimulation/inhibition (Item #35150-637)
    1.5 mW/mm2, with a 625 nm short-pass dichroic filter.
 
  • Co-axial optical mount to pre-existing optical imager.
  • Tandem lenses: 25 mm & 50 mm.
  • Field of view: Fixed at 10 mm X 10 mm (1024 X 1024).
  • Easily switch from one wavelength to the other by swapping the magnetic filter mount.
  • nHD (360 X 360 pixels) light modulator in a 8.5 mm X 8.5 mm area.
  • 100 frames per second.
  • Other wavelength available on requests, multiple wavelength possible.
lighttrack ois200 banner

Dual Camera imaging (Item #35250)

  • Allows fluorescence imaging of 2 fluorophores simultaneously, combined with intrinsic signal imaging for hemodynamic correction. Proper optical filter selection is required and must be discussed.
  • Doubled frame rate.
  • Tandem lenses: 25 mm & 50 mm.
  • Field of view: Fixed at 10 mm X 10 mm (1024 X 1024).
  • 2X teleconverters (sold separately) can be added to obtain a 10 mm X 10 mm field of view on 512 X 512 pixels, for high speed imaging.
  • Same field of view (10% offset corrected by co-registration in our analysis scripts).
 

Accessories

behavioral camera on white background

Behavioral Camera (Item #33011)

  • Integrated to the acquisition software for precise synchronization (0.1 ms) with the main imaging camera. 
  • Adjustable binning, region of interest, exposure time and frame rate. 
  • 1024 x 1024 at 30 fps, 256 x 128 at 900 fps.

Light Tight Enclosure with Black Curtain in Front for Easy Access (Item #33001)

  • Dimensions: 26″ x 26″ x 26″.
  • Systems equipped with the spatially modulated optogenetic stimulation add-on: Height is 32″.
light tight box with labeo tech logo

Light Tight Enclosure with Front Door Opening Upward (Item #33002)

  • Dimensions: 26″ x 26″ x 26″.
  • Systems equipped with the spatially modulated optogenetic stimulation add-on: Height is 32″.
drop in t slot nuts
base of the ois200

Drop-in T-Slot Nuts (Item #33101)

  • Content: 5x M6 & 5x 1/4-20
  • These nuts can be installed in the T-Slot rails on both sides of the system.
  • Once installed, they allow the user to screw any hardware onto the imaging system, such as a monitor for visual stimulation or a custom-built enclosure.
post holder for the ois system
clip mounted on the ois200

Post Holder (Item #33102)

  • Can be installed onto both sides of the imaging system.
teleconverter lens
teleconverter on the ois200

2X Teleconverter (Item #33051)

  • For systems equipped with the optogenetic stimulation add-on and dual camera imaging only. It allows changing the focal length of the bottom lens by 2x, offering a field of view 2 times larger. This is especially useful at high frame rate and limited ROI. 2 teleconverters can be attached for a field of view 4 times larger.
eclipse side view

Magnetic Stand (Item #33151)

  • Spot size down to 50 µm, up to 250 mW/mm2 power output.
  • 2D Illumination Scanning with galvo mirrors.
  • Co-axial optical mount to pre-existing optical imager.
  • Tandem lenses: 25 mm & 50 mm.
  • Field of view: Fixed at 10 mm X 10 mm (1024 X 1024).
  • 2X teleconverters (sold separately) can be added to obtain a 10 mm X 10 mm field of view on 512 X 512 pixels, for high speed imaging.
  • Dichroic filter to ensure compatibility with existing applications.
  • Easily switch from one wavelength to the other by swapping the magnetic filter mount.
  • Multi-points stimulation: 2 points or matrix selection.
  • Pre-conditioning with adjustable laser power and inter-stimulation duration: fixed, ramps or list.
  • Pulsed stimulation up to 500 Hz.

Items Included With the System

ois200 illumination pod
ois200 illumination pod with 6 lazers

Illumination Pod

  • Manually control the illumination intensity for each laser color.
  • Dimensions: 120mm x 80mm x 45mm
  • Configuration varies depending on the imaging options selected.
asus monitor with a computer fixed to the the back

Computer and Monitor (Item #33101)

  • High-performance computer fixed behind the monitor with acquisition software and drivers pre-installed.
  • Windows 10 Pro, AMD Ryzen 4000 processor, 1 TB SSD, 32GB DDR4, 23” monitor.
  • Configuration may vary depending on availability.
  • Wireless mouse and keyboard.
  • This item is included but optional, instructions can be sent to the client to install the software on a compatible computer. The computer must have at least 16 GB DDR4 (32 GB recommended) and a SSD drive as the main C drive (the drive must not use QLC technology, such as Intel’s Optane or 660p series). Contact us for compatibility confirmation.
screenshot of the ois200 user interface

Acquisition Software

  • Adjustable frame rate, exposure time, binning, ROI, illumination selection and stimulation parameters.

Generic Analysis Software

  • Generic analysis software and user interface in Matlab to open the data and display typical images and videos such as HbO, HbR and relative blood flow maps (only with the Speckle option). Open source for easy modifications for customers needs. Matlab software and toolboxes not included.

Pictures of the System

ois200 manual focus button

Focus Adjustment

  • A translation stage is fixed on the camera for systems without the optogenetic stimulator or dual camera imaging add-on.
  • A lab jack is included with the add-ons.
height adjustment on the ois200

Height and Inclination Adjustment

  • Height can be adjusted by up to 13 cm.
  • Inclination can be adjusted by up to ±30 degrees.
mounting points of the ois 200

Multiple Mounting Points for Illumination Arms

  • 4 mounting points for the illumination arms are present on the on the imaging arm. They move with the camera (height and inclination).
ois200 dimensions

General Dimensions

  • Dimensions are shown in the pictures beside.
ois200 arms mounted backwards

Imaging Arm Mounted Backwards

  • For imaging of large animals, such as monkeys.